人去唾液酸糖蛋白受體(AGSPR)ELISA檢測試劑盒英文名稱Human asialoglyco protein receptor,AGSPR ELISA試劑盒齊一生物科技有限公司主要經(jīng)營進口原裝Elisa試劑盒,種類齊全,價格實惠,貨期短.購買我公司產(chǎn)品新老客戶,可享全程技術(shù)指導(dǎo)：(包括售前標(biāo)本收集、后期實驗、實驗結(jié)束后數(shù)據(jù)分析等技術(shù)問題

人去唾液酸糖蛋白受體(AGSPR)ELISA檢測試劑盒產(chǎn)品規(guī)格：96T/48T
產(chǎn)品庫存：現(xiàn)貨
產(chǎn)品價格：詢價
使用范圍：僅供科研檢測,不得用于臨床.
保存條件：2-8℃
發(fā)貨方式：專門快遞免費送貨上門
產(chǎn)品價格：*,洽談！
說明書:說明書隨貨發(fā)送,您也可以直接我司在線銷售人員索取.全國：代理多種品牌進口原裝、分裝“ELISA試劑盒".齊一生物科技有限公司作為酶聯(lián)免疫供應(yīng)商,憑借*的技術(shù),*的售前,售中,售后免費代測服務(wù),共同鑄就高品質(zhì)的產(chǎn)品.多年專業(yè)酶免服務(wù),質(zhì)量保證,可免費提供代測服務(wù),一周內(nèi)出結(jié)果.咨詢訂購.
ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù).每個標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔.每個樣品根據(jù)自己的數(shù)量來定,能使用復(fù)孔的盡量做復(fù)孔.標(biāo)本用標(biāo)本稀釋液1：1稀釋后加入50ul于反應(yīng)孔內(nèi).
加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測樣品50ul于反應(yīng)孔內(nèi).立即加入50ul的*標(biāo)記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機洗滌,洗滌次數(shù)增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測定結(jié)果.
在450nm波長處測定各孔的OD值.
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9011708    Cable, LSB to AC, BR8000        2,450.00
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9013035    Top, auto vac, BR8000        18,730.00
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130701    NeXtal Tubes Classics Suite        8,780.00
130712    NeXtal Tubes MBClass II Suite        8,780.00
990390    Reagent Bottle Rack        339.00
990395    Starter Pack, QIAcube        7,850.00
76405    Allprotect Tissue Reagent Kit (100ml)    100 ml Allprotect Tissue Reagent, Allprotect Reagent Pump    8,290.00
9019877    Cover, main, blue, RG6        4,120.00
9010854    Motor, gripper V and handler V        13,670.00
9011277    Plate, ground, auto vac, BR8000        8,390.00
9011833    Dilutor unit, BR8000        54,800.00
9012397    Holder, vacuum trap, auto vac, BR8000        146,510.00
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9013036    Auto vac unit, BR8000        77,550.00
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130716    NeXtal Tubes PEGs II Suite        8,780.00
30765    Ni-NTA Superflow Cartridges (100x5 ml)        114,700.00
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55114    QIAamp Circulating Nucleic Acid Kit (50)        14,450.00
9019731    Board, A/D, V2, QX        26,610.00
950133    Binding Reagent (DXB) (350 ml)        1,720.00
70022    Oligotex mRNA Mini Kit    For 12 mRNA minipreps: 200 ul Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents and Buffers    4,040.00
74904    RNeasy Plant Mini Kit (50)    50 RNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers    4,650.00
79254    RNase-Free DNase Set (50)    1500 units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps    1,370.00
120018    QIAquick Buffer Module 8000        6,270.00
203203    HotStarTaq DNA Polymerase (250)     - 250 units HotStarTaq DNA 人去唾液酸糖蛋白受體(AGSPR)ELISA檢測試劑盒Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2    1,810.00
301105    PolyFect Transfection Reagent (1.0 ml)    For 25-65 transfections in 60 mm dishes or 50-100 transfections in 6-well plates.    1,490.00
1005485    Q-Solution,5 x (2ml/2)        67.30
9015636    Fuse-2, 3.15A (for PSB board), M48        659.00
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132630    NeXtal Stock Zinc acetate (50)    1M Zinc acetate dihydrate - 50mL (4 x 12,5 ml tube)    737.00
9019575    Opto switch, X-axis, Cxx        249.00
9019605    Pipettor, spring guard ring (black), Cx2        211.00
9019621    Tip ejector holder (incl. magnet), Cx2        211.00
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