詳細介紹
骨形成蛋白-2(BMP-2)檢測試劑盒英文名稱Human Bone morphogenetic protein 2.(BMP-2) ELISA Kit齊一生物科技(上海)有限公司是一家專業(yè)銷售酶聯(lián)免疫試劑盒,抗體蛋白、生化試劑,標準品和對照品的大型化學科技公司.自公司成立以來,本著始終擁有的創(chuàng)業(yè)激情,公司的銷售額保持高速增長,企業(yè)規(guī)模不斷擴大.齊一生物科技(上海)有限公司全國
骨形成蛋白-2(BMP-2)檢測試劑盒英文操作步驟
實驗開始前,請?zhí)崆芭渲煤盟性噭噭┗驑悠废♂寱r,均需混勻,混勻時盡量避免起泡。每次檢測都應該做標準曲線。如樣品濃度過高時,用樣品稀釋液進行稀釋,以使樣品符合試劑盒的檢測范圍。
1. 加樣:分別設空白孔、標準孔、待測樣品孔。空白孔加樣品稀釋液100μl,余孔分別加標準品或待測樣品100μl,注意不要有氣泡,加樣將樣品加于酶標板孔底部,盡量不觸及孔壁,輕輕晃動混勻,酶標板加上蓋或覆膜,37℃反應120分鐘。
為保證實驗結果有效性,每次實驗請使用新的標準品溶液。
2. 棄去液體,甩干,不用洗滌。每孔加*標記抗體工作液 100μl(取1μl*標記抗體加99μl*標記抗體稀釋液的比例配制,輕輕混勻,在使用前一小時內(nèi)配制),37℃,60分鐘。
3. 溫育60分鐘后,棄去孔內(nèi)液體,甩干,洗板3次,每次浸泡1-2分鐘,350μl/每孔,甩干。
4. 每孔加辣根過氧化物酶標記親和素工作液(同*標記抗體工作液) 100μl,37℃,60分鐘。
5. 溫育60分鐘后,棄去孔內(nèi)液體,甩干,洗板5次,每次浸泡1-2分鐘,350μl/每孔,甩干。
6. 依序每孔加底物溶液90μl,37℃避光顯色(30分鐘內(nèi),此時肉眼可見標準品的前3-4孔有明顯的梯度藍色,后3-4孔梯度不明顯,即可終止)。
7. 依序每孔加終止溶液50μl,終止反應(此時藍色立轉黃色)。終止液的加入順序應盡量與底物液的加入順序相同。為了保證實驗結果的準確性,底物反應時間到后應盡快加入終止液。
8. 用酶聯(lián)儀在450nm波長依序測量各孔的光密度(OD值)。 在加終止液后15分鐘以內(nèi)進行檢測。
Phospho-53BP1 (S6) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Abl1/2 (Y393/439) Cell-Based Fluorometric ELISA Kit Human, Mouse
Phospho-Abl1/2 (Y393/439) Cell-Based Fluorometric ELISA Kit Human, Mouse
Phospho-ACCα (S80) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-FoxO4 (S197) Cell-Based Fluorometric ELISA Kit Human, Mouse
Phospho-Akt (S473) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Akt (T72) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Akt (Y474) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Akt (T308) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Akt1 (T450) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Akt2 (S474) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-AMPKα1 (S487) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-AR (S650) Cell-Based Fluorometric ELISA Kit Human
Phospho-AR (S94) Cell-Based Fluorometric ELISA Kit Human, Mouse
Phospho-AQP2 (S256) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-ATF-2 (T69) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-ATF-2 (S62) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-ATF-2 (T71) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-ATF-2 (T73) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Na+/K+-ATPase α1 (S23) Cell-Based Fluorometric ELISA Kit Rat
Phospho-Na+/K+-ATPase α1 (S16) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Bad (S136) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Bad (S155) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Bcl-2 (S70) Cell-Based Fluorometric ELISA Kit Human
Phospho-Bcl-2 (S87) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Bcl-2 (T56) Cell-Based Fluorometric ELISA Kit Human
Phospho-Bcl-x (S62) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Bcr (Y177) Cell-Based Fluorometric ELISA Kit Human, Mouse
Phospho-Raf-B (S602) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-BRCA1 (S1423) Cell-Based Fluorometric ELISA Kit Human, Rat
Phospho-BRCA1 (S1457) Cell-Based Fluorometric ELISA Kit Human
Phospho-BRCA1 (S1524) Cell-Based Fluorometric ELISA Kit Human
Phospho-Btk (Y223) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
英文Phospho-Btk (Y551) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-C/EBP α (S21) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-C/EBP α (T226) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-C/EBP β (T235) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-CaMKIIα/δ (T286) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-CaMKIV (T200) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Caspase-8 (S347) Cell-Based Fluorometric ELISA Kit Human, Rat
Phospho-Caspase-8 (Y380) Cell-Based Fluorometric ELISA Kit Human
Phospho-Caspase-9 (T125) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Catenin-β (T41/S45) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Caveolin-1 (Y14) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-CD88 (S338) Cell-Based Fluorometric ELISA Kit Human
Phospho-Cdk2 (T160) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Chk1 (S286) Cell-Based Fluorometric ELISA Kit Human
Phospho-Chk1 (S296) Cell-Based Fluorometric ELISA Kit Human
Phospho-Chk1 (S301) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Chk2 (T68) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-AP-1 (S243) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-AP-1 (S63) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-AP-1 (S73) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-AP-1 (T231) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-AP-1 (T239) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-AP-1 (T91) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-AP-1 (T93) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-AP-1 (Y170) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
Phospho-Claudin-5 (Y217) Cell-Based Fluorometric ELISA Kit Human, Mouse, Rat
β淀粉樣肽(1-40),超純,HCl
β淀粉樣肽(1-40), Starter Kit
β淀粉樣肽(1-40), Starter Kit
β淀粉樣肽(1-42),超純,TFA
β淀粉樣肽(1-42),超純,TFA
β淀粉樣肽(1-42),超純,HFIP
β淀粉樣肽(1-42),超純,HFIP
β淀粉樣肽(1-42),超純,NaOH
β淀粉樣肽(1-42),超純,NaOH
β淀粉樣肽(1-42),超純,HCl
β淀粉樣肽(1-42),超純,HCl
β淀粉樣肽(1-42), Starter Kit
β淀粉樣肽(1-42), Starter Kit
β淀粉樣肽(1-43),超純,TFA
β淀粉樣肽(1-43),超純,TFA
β淀粉樣肽(1-40),小鼠,大鼠
β淀粉樣肽(1-42),小鼠,大鼠
不規(guī)則β淀粉樣肽(1-40)
不規(guī)則β淀粉樣肽(1-40)
不規(guī)則β淀粉樣肽(1-42)
不規(guī)則β淀粉樣肽(1-42)
β淀粉樣肽(1-40,F4W)
β淀粉樣肽(1-40,F4W)
β淀粉樣肽(1-40,Y10W)
β淀粉樣肽(1-40,Y10W)
β淀粉樣肽(1-40,D23N)
β淀粉樣肽(1-40,D23N)
β淀粉樣肽(1-42,M35V)
β淀粉樣肽(1-42,M35V)
β淀粉樣肽(1-42,R5G)
β淀粉樣肽(1-42,R5G)
β淀粉樣肽(1-42,Y10A)
β淀粉樣肽(1-42,Y10A)
β淀粉樣肽(1-42,F(xiàn)4W)
β淀粉樣肽(1-42,F4W)
β淀粉樣肽(1-42,H6A)
β淀粉樣肽(1-42,H6A)
β淀粉樣肽(1-42,H13A)
β淀粉樣肽(1-42,H13A)
β淀粉樣肽(1-40,H14A)
β淀粉樣肽(1-11)
β淀粉樣肽(1-11)
β淀粉樣肽(1-16)
β淀粉樣肽(1-16)
β淀粉樣肽(1-28)
β淀粉樣肽(1-28)
β淀粉樣肽(11-22)
β淀粉樣肽(11-22)
β淀粉樣肽(1-40)
β淀粉樣肽(1-40),小鼠,大鼠
β淀粉樣肽(12-28)
β淀粉樣肽(12-28)
β淀粉樣肽(17-40)
β淀粉樣肽(17-40)
β淀粉樣肽(17-42)
β淀粉樣肽(17-42)
β淀粉樣肽(22-35)
β淀粉樣肽(22-35)
β淀粉樣肽(25-35)
β淀粉樣肽(25-35)
15N β淀粉樣肽(1-40)
15N β淀粉樣肽(1-40),大鼠
15N β淀粉樣肽(1-42)